The cytotoxic effects of titanium dioxide nanoparticles on MCF-7 cancer cell line

Iranian Journal of Veterinary Science and Technology

مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 18 تیر 1403   

نوع مقاله: Research Article

شناسه دیجیتال (DOI): 10.22067/ijvst.2024.86303.1340

نویسندگان

Rahele Javaheri¹؛ Ahmad Reza Raji^{* 2}؛ Hadi Mohebalian³؛ Mohammad Azizzadeh⁴

¹comparative Histology, Faculty of veterinary medicine, Ferdowsi University of Mashhad, Iran

²Ferdowsi University of Mashhad

³Department of Pathobiology, Faculty of Veterinary medicine, Ferdowsi University of Mashhad, Iran

⁴Department of Clinical Sciences, Faculty of Veterinary medicine, Ferdowsi University of Mashhad, Iran,

چکیده

Cancer is a widespread disease worldwide, affecting many people with various types. Today, titanium dioxide nanoparticles have substantial therapeutic applications. We conducted a study to see how harmful titanium dioxide is to breast cancer cells.

MCF-7 cancer cells and HFF cell lines were cultured. We tested how cells survived when exposed to different amounts of titanium dioxide nanoparticles. The concentrations examined have been 25, 50, 100, and 200μg/ml. We measured the survival rate at 48, 72 hours and determined the IC50.

We determined that the rate toxicity occurred while MCF-7 and HFF cells had been exposed to 200μg/ml of titanium dioxide. Apoptosis in MCF-7 and HFF cells emerges as shown with the aid of way of Annexin V-PI staining and go with the flow cytometry. Under a microscope, it was found that titanium dioxide nanoparticles can be harmful in specific amounts. At a dose of 200μg/ml, after 48 and 72 hours of treatment, MCF-7 and HFF cells were affected. The mitochondrial membrane broke when breast cells were exposed to titanium dioxide nanoparticles. The matrix leaked into the cytoplasm, and the rough endoplasmic reticulum swelled. These observations occurred after 72 hours of treatment with a concentration of 200 µg /ml.

In keeping with the acquired effects, titanium dioxide nanoparticles may be advocated as potential medicinal candidates for pharmaceutical purposes, even though further research is required.

کلیدواژه‌ها

TiO2 Nanoparticles؛ Flow Cytometry؛ Micronucleus Assay؛ MTT Assay؛ TEM